In this present investigation, the authors obtain Fekete-Szegö's inequality for certain normalized analytic functions defined on the open unit disk for which lies in a region starlike with respect to and is symmetric with respect to the real axis. Also certain applications of the main result for a class of functions defined by convolution are given. As a special case of this result, Fekete-Szegö's inequality for a class of functions defined through fractional derivatives is obtained. The Motivation of this paper is to give a generalization of the Fekete-Szegö inequalities obtained by Srivastava and Mishra .;